1. Summary information’s

Please view the table below for a summary of currently accepted article types and general manuscript guidelines.

Type of Submission	Abstract Length (max)	Figures and tables (combined, max)	Manuscripts Length (words)	Peer review
Research Paper	250 words	15	12 000	yes
Minireview	250 words	2	3 000	yes
Case Report	350 words	4	3 000	yes

 2. Manuscript Guidelines

 2.1. Registration with Applied Biosciences

Please note that the corresponding author MUST register with Applied Biosciences before submitting an article. You must be logged in to your personal Applied Biosciences Account to submit an article.

3. Manuscript Requirements and Guide Style

 3.1. General Requirements 

• Font, size and file format

In Applied Biosciences the default font is Times New Roman with the size of 12. Within the text provide the row numbers. The entire documents should be single spaced. Your manuscript should be written using either LaTeX or MS-Word. The preferred file format for manuscripts is *.doc, *.docx, or *.pdf.

• Sections

Your manuscript should be organized by headings and subheadings.

Original Research Articles should be organized in the following sections:

• Introduction:
  • Condense representation of current knowledge, with no subheadings.
• Material and Methods:
  • This section should contain sufficient detail so that when reading in conjunction with cited references, all procedures can be repeated. For experiments reporting results on animal or human subject research, an ethics approval statement should be included in this section. This section can be divided by subheadings.
• Results:
  • Full representation of the obtained results. This section can be divided by subheadings.
• Discussion:
  • Discussions should briefly cover the key findings of the study: discuss any findings related to the subject so to bring out the novelty of the discovery; discuss their integration into the current understanding of the problem and how this advances the current views; you may freely postulate theories that could be tested in the future. This section can be divided by subheadings.

Minireview should be organized in the following sections:

• Introduction/Opinion:
  • A comprehensive representation of current knowledge, with possible subheadings.
• Conclusions with future outlook:
  • Discussions should briefly cover the key findings of the study and speculate on the future direction of the research and freely postulate theories that could be tested in the future.

For Case Reports the following sections are mandatory:

• Introduction:
  • Include symptoms at presentation, physical exams, and lab results.
• Background:
  • This section may be divided by subheadings. Include history and review of similar cases.
• Discussion:
  • This section may be divided by subheadings. Include diagnosis and treatment.
• Concluding Remarks

3.2. Language Style

The default language style at Applied Biosciences is American English.

3.3. Title

 The title should be written in bold and centered at the top of the first page. The title should be succinct, and be a statement of the main result or conclusion presented in the manuscript. Creative titles are welcome, but only if relevant and within the proper measure. Authors should try to avoid if possible titles that are a mere question without giving the answer or unambitious and abbreviations within the title.

 3.4. Authors and Affiliations

 All names are listed together under the title and separated by commas. Provide exact and correct author names (first and last) to avoid indexing issues. Affiliations should be linked to the author's name with superscript numbers and be listed as follows: Laboratory, Institute, Department, Organization, City, State abbreviation (USA, Canada, Australia), and Country (without detailed address information such as city zip codes or street names).

Example: John Doe, Department of Neurology, International University of Knowledge, New York, NY, USA.

The Corresponding Author(s) should be marked with an asterisk. Provide the exact email address of the corresponding author(s) in a separate section.

 3.5. Abstract

 The abstract should represent the general significance and conceptual advance of the work accessible to a broad readership. Minimize the use of abbreviations and do not cite references. See Summary Table for abstract requirement and length according to article type.

 3.6. Keywords

 Four keywords are mandatory; eight is maximum.

4. Detailed Guidelines

4.1. Nomenclature

The use of abbreviations should be kept to a minimum. Non-standard abbreviations should be defined upon first use in the main text. Equations should be inserted in editable format from the equation editor. Gene symbols should be italicized; protein products not. Chemical compounds and biomolecules should be referred using the recommendations by IUPAC. We request the use of Standard International Units in all manuscripts.

 4.2. Figure and Table Guidelines

We strongly encourage to submit the figures in your article with the highest possible quality. It is important to submit digital art that conforms to the appropriate resolution, size, and file format. Figures and Tables Titles should be placed at the end of the manuscript, after a list of references.

General Information:

• All figures should be provided as separate files, which will be uploaded separately during submission. Do not submit figure files embedded in to document.
• All figures are numbered according to its sequence in the text. All figures must have captions.
• Do not include figure numbers, captions, or author names as part of the figure.
• When uploading a figure file to Submission Portal, make sure to copy and paste the figure caption into the caption field.
• For multipaneled figures, they must be submitted assembled. Label identifications should appear in 14 pt Arial font in bold. Please maintain the same font for all figures submitted with your manuscript.
• Capitalize only the first word and proper nouns in each label.
• Scale bars should be inserted to indicate magnification.
• Photographic images should be clear and of high quality, cropped at right angles.

File Sizes and Format:

• 1 column: 85 mm minimum 1050 pixels wide
• 2 column: 178 mm minimum 2100 pixels wide
• The maximum height of a figure is 246 mm
• We accept the following figure file formats: *.tif, *.jpg, or *.eps are preferred. When saving TIFF files, use only LZW compression; do not use JPEG compression. If images are submitted in color, they should be submitted in RGB format.

We recommend usage of graphics programs while preparing figures e.g.: Inkscape or Adobe Illustrator.

 4.3. Supplementary Files

Supplementary materials can consist of tables, figures, movies, lists, or external links. Acceptable file formats are .jpg, .tif, .eps for image files, and Word, Excel, files for texts and databases. For Original Research Articles, you may upload maximum 15 Supplementary Files.

 4.4. Code

If you wish to submit a code with your manuscript, it should follow appropriate coding style, should be novel and presented in a human-readable format as a Supplementary File. Source code should be well documented (comments in source), be provided with an example data set to show efficacy, be compilable or executable free of errors.

 4.5. Data Availability Statements

While publishing with Applied Biosciences authors are obliged to include a data availability statement detailing where the data supporting the conclusions of the manuscript can be found. 

 4.6. Statistics

Papers published in Applied Biosciences are diverse, and it is not possible to give specific statistical recommendations, guidelines, or rules that can apply to all manuscripts. General guidelines and recommendations that are given here are relevant for a large number of situations. Always specify the experimental design such as: as a number of replications, blocks, repeated measurements, samples, or observations. Except for simple procedures (e.g. t-tests, one-way analysis of variance, simple linear regression analysis), cite an appropriate and accessible statistical text and indicate the computer software used. It is not sufficient to just mention the name of a large and multifaceted software package such as SAS, SPSS, or R. List the specific procedure or package used (e.g., PROC MIXED in SAS, or the lme4 package in R). In general, statistical techniques should be described in the Materials and Methods. We recommend verifying your statistics with a professional Statistician. 

 4.7. References

All citations in the text, figures or tables must be in the reference list and vice-versa. The references should only include articles that are published or accepted. For accepted but unpublished works use "in press" instead of page numbers. Unpublished data, submitted manuscripts, or personal communications should be cited within the text only. Use the author-year method of citing publications. For example:

Within the body of the text: Johnas et al., 2012

Within the List of References: Johnas M., Mathiews D., Kowalski J., and Nowak A. (2012); The meaning of life; App Biosc;  26; 235-251

 5. Additional Content

5.1. Acknowledgments

The submitters may write optional, short text to acknowledge the contributions of specific colleagues, institutions, or agencies that aided the efforts of the authors.

 5.2. Author Contributions Statement

The Author Contributions Statement may be up to several sentences long and should fleetingly describe the tasks of the individual authors. Please list only 2 initials for each author, without spaces (e.g. JC, JS). In the case of two authors with the same initials, please use their middle initial to differentiate between them (e.g. REW, RSW). The Author Contributions Statement should be included at the end of the manuscript before the References.

 5.3. Conflict of Interest Statement

A Conflict of Interest Statement needs to be included at the end of the manuscript before the references. Here, the authors need to declare whether or not the submitted work was carried out in the presence of any personal, professional or financial relationships that could potentially be interpreted as a conflict of interest.

 5.4. Funding disclosure

Authors of manuscripts submitted to Applied Biosciences are expected to list all sources of funding for the research project at the time of submission.

 6. Before submitting

6.1. Cover Letter

A cover letter must be included in the submission, which should briefly (3-5 sentences) explain the significance of the research. Please include the title of the manuscript together with its unique submission number.

 6.2. Authors Checklist

Check here your author's checklist.

 7. Upon publication

7.1. Uncorrected proof

Proofs of edited articles are made available to the corresponding author via e-mail and Submission Portal. The article page proofs must be reviewed.

Adobe Reader, version 7 or higher, will be needed to read and annotate the electronic proofs in the Review Mode. If authors do not already have access to Adobe software, a link for downloading a free version of the Adobe Reader can be found in the e-mail accompanying the proofs.

 7.2. Fees

For publishing with Applied Biosciences the Article Processing Charge (APC) of 500 EUR will be required, regardless of the manuscript size and after acceptance of the manuscript. Payment can be done with a wire transfer or PayPal. As an Applied Biosciences Reviewer, you are qualified to receive a discount.

8. Journal Services

8.1 Language Correction

If you are a non-native English speaker, you can use English correction service of your submitted work. The payment depends on the level of English presented in your work, however the maximum payment will be not more than 100 EUR.

The Language Correction Service is provided by designated workers of our Publisher: Institute of Biotechnology and Molecular Medicine Foundation (Visit Website), not by Applied Biosciences members of Editorial Office.